Protein stability instrument/NanoTemper/Prometheus NT.48

Vienna Biocenter Core Facilities GmbH (VBCF)

Wien | Website

Large equipment

Short Description

The NanoTemper Prometheus is a multi-purpose, label-free instrument measuring both intrinsic protein fluorescence and scattering while applying a defined temperature profile. Structural changes (folding/unfolding) as well as aggregation can be monitored over time, as a function of temperature, or under different chemical conditions. Since a sensitive fluorescence detection setup is used, only a minute protein sample is needed (>5µg/ml, 10µl). The Prometheus serves not only as a quality control instrument but also as a tool to find optimum conditions by using buffer screens to optimize stability. Using the Prometheus as a tool for day-to-day protein quality checks will improve the reproducibility of subsequent experiments that use purified proteins for structural and interaction studies while screening for potential binders can open up subsequent avenues of investigation.

https://nanotempertech.com/prometheus/

Contact Person

David Drechsel

Research Services

Please refer to: https://www.viennabiocenter.org/vbcf/protein-technologies/

Methods & Expertise for Research Infrastructure

Please refer to: https://www.viennabiocenter.org/vbcf/protein-technologies/

Allocation to Core Facility

Protein Technologies

David Drechsel
Vienna Biocenter Core Facilities (VBCF)
+43 1 7962324 7070
david.drechsel@vbcf.ac.at
https://www.viennabiocenter.org/vbcf/protein-technologies/
Vienna BioCenter Core Facilities (VBCF) offer state-of-the-art research infrastructure and scientific services to academic research institutes and companies in the field of Life Sciences at Vienna BioCenter and beyond. Our general cooperation conditions are available at https://www.viennabiocenter.org/vbcf/ (Downloads – VBCF General Cooperation Conditions). Information on usage fees is provided upon request by the core facilities.