Spinning Disk Confocal Laser Microscope

University of Veterinary Medicine Vienna

Wien | Website

Large equipment

Short Description

Spinning disk confocal laser microscope with incubation chamber for fast and sensitive imaging of fluorescently labeled fixed and live specimens.

-Fully motorized Nikon Eclipse Ti2-E
-Yokogawa CSU-W1 dual disk (25 µm, 50 µm), dual camera port
-Laser lines: 405, 488, 561, 638 nm
-Lumencor Spectra III LED light engine
-Large format (25 mm FOV) Nikon DS-Qi2 CMOS camera
-Two Photometrics Prime BSI sCMOS cameras, 95% QE, 6.5 µm x 6.5 µm pixel area
-Widefield and confocal multiple position imaging, tile scanning, z-stack acquisition
-Incumbation chamber with controllable temperature and CO2

Contact Person

Martin Glösmann

Research Services

Support with experimental design and image acquisition, single and multichannel confocal microscopy, tile scanning, z-stack and time series acquisition, data analysis and visualization

Methods & Expertise for Research Infrastructure

Confocal microscopy
Multi-fluorochrome detection
Z-stack and time series acquisition
Deconvolution, 3D-Rendering, image analysis

Allocation to Core Facility

VetCore Technologiezentrum der Vetmeduni Wien

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