University of Salzburg
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Short Description
Fixed samples provide only a brief snapshot of cellular processes. By observing living cells or organisms, one can gain insights into the dynamics and interaction of proteins, organelles and cells. The AxioObserver (Zeiss) is suitable for transmitted light brightfield, phase contrast, DIC, PlasDIC and incident light fluorescence and is equipped with a temperature control device and motorized object table. The microscope can be used for fluorescence-based analysis of fixed and living cells in the field of cell biology and cancer research.
Contact Person
Prof. Dr. Silja Wessler
Research Services
Live Cell Imaging
Methods & Expertise for Research Infrastructure
The device is used in various molecular biology disciplines such as molecular tumor biology and molecular immunology research. With the help of fluorescence-based labeling methods, the device enables the analysis of fixed as well as living cells, a central technology and prerequisite for the development of highly cited publications of CF tumor biology. Equipment: Transmitted light bright field, phase contrast, DIC, PlasDIC and incident light fluorescence with a temperature control device and motorized object table
Allocation to Core Facility
2008-2017
Josef Thalhammer: Koordinator
Fonds zur Förderung der wissenschaftlichen Forschung
Apoptosis or Cell Survival: c-Abl in Helicobacter pylori-infected epithelial cells
2012-2017
Silja Wessler
Fonds zur Förderung der wissenschaftlichen Forschung, P24315
Targeting E-cadherin - Functional role of E-cadherin shedding in Helicobacter pylori-associated carcinogenesis
2012-2017
Silja Wessler
Fonds zur Förderung der wissenschaftlichen Forschung, P24074
2013
Posselt G, Backert S, Wessler S
Cell Communication and Signaling
DOI: 10.1186/1478-811X-11-77
Helicobacter pylori HtrA is a new secreted virulence factor that cleaves E-cadherin to disrupt intercellular adhesion.
2010
Hoy B, Löwer M, Weydig C, Carra G, Tegtmeyer N, Geppert T, Schröder P, Sewald N, Backert S, Schneider G, Wessler S.
EMBO Reports
DOI: 10.1038/embor.2010.114
CagA phosphorylation in Helicobacter pylori-infected B cells is mediated by the non-receptor tyrosine kinases of the Src and Abl families.
2016
Krisch LM, Posselt G, Hammerl P, Wessler S
Infection and Immunity
DOI: 10.1128/IAI.00349-16
Identification of E-cadherin signature motifs functioning as cleavage sites for Helicobacter pylori HtrA
2016
Schmidt TP, Perna AM, Fugmann T, Böhm M, Jan Hiss, Haller S, Götz C, Tegtmeyer N, Hoy B, Rau TT, Neri D, Backert S, Schneider G, Wessler S.
Scientific reports
DOI: 10.1038/srep23264.
E-Cadherin Orthologues as Substrates for the Serine Protease High Temperature Requirement A (HtrA)
2022
Bernegger S, Hutterer E, Zarzecka U, Schmidt TP, Huemer M, Widlroither I, Posselt G, Skorko-Glonek J, Wessler S.
Biomolecules
DOI: 10.3390/biom12030356