FEI ESEM XL30 Scanning electron microscopy

University of Salzburg

Salzburg | Website

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Short Description

The FEI ESEM XL30 is a scanning electron microscope with variable pressure control and X-ray microanalysis. It is versatile in biological research. The maximum resolution, the device operated with a tungsten cathode, is a maximum of 3.5nm. The acceleration voltage can be varied in the range of 0.2 - 40 kV.

Contact Person

aoProf. Mag. Dr. Bernd Minnich

Research Services

Biomedicine
Reproductive biology
Forensic medicine
Protozoology
Sports medicine
Vascular biology

Methods & Expertise for Research Infrastructure

-Vascular- and Tumors Research: Investigation of vascular corrosion casts with respect to the growth and development of blood vessels in organs and tissues.
-Analysis of the vasa vasorum, the large human vena saphena with vascular corrosion casts and its investigation using the 3D -morphometry system M3. Morphometric / stereological analyzes in all areas mentioned;
-Various fine structure studies on artropoda in the taxonomic and ecological context.

A central research area is also the fine-structure analysis of cellular defense processes in neutrophil granulocytes-mediated inflammatory reactions in chronic diseases of humans, especially with regard to formation NETs (neutrophil extracellular traps). This affects diseases such as cystic fibrosis (mucoviscidosis), COPD and periodontal disease (also associated with rheumatoid arthritis).

In the field of material research, fracture analyzes of material parts are carried out.

Allocation to Core Facility

Advanced Immaging and Microscopy

aoProf. Mag. Dr. Bernd Minnich
Fachbereich Biowissenschaften
0043 662 8044 5607
bernd.minnich@sbg.ac.at
https://www.uni-salzburg.at/index.php?id=208727
Please contact us via science.plus@sbg.ac.at, or contact the responsible person for this section, mentioned in the contact field
Prof. Dr. Studnicka, Pulmology, SALK, Salzburg
Dr. Marcos, V. Research center Childrens Hospital, LMU Munich
Prof. Dr. Hartl D., University TübingenChildren`s Hospital and Interdiciplinary Center for infectious Diseases
Prof. Dr. Hannik, University Clinic Homburg an der Saar
Dr. Vitkov, Parodontologe
Prof. N. Hüsing, Department of Chemistry and Physics of Materials, University Salzburg
3rd Medicine, Charles University Praha, Prof. Kachlik / Prof. Stingl
PMU Salzburg, Urology, Dr. Foditsch
PMU Salzburg, Interventional Neuroradiology, Prof. Killer
Untersuchung der Lungenvaskularisation bei COPD / Emphysemen im Mausmodell - Vascular Corrosion casting
2015
Bernd Minnich, Katharina Erlbacher
Koop.Partner: Dr. Max Ackermann, Inst. f. Anatomie, Med.UNI Mainz, D

Untersuchung der gonadalen Vaskularisation unter Einfluss von Cannabis (THC) im Rattenmodell - Vascular Corrosion casting
2013-2015
Bernd Minnich, Katharina Erlbacher
Koop.Partner: Prof. Paulmichl, Pharmakologie & Toxikologie, PMU
Stiftungs- und Förderungsgesellschaft, Universität Salzburg - Forschungsprojekt: SF0314M

Untersuchung der penilen Vaskularisation bei Diabetes II im Rattenmodell - Vascular Corrosion casting
2015-2017
Bernd Minnich, Katharina Erlbacher
Koop.Partner: Dr. Esra Foditsch, Urologie, SALK - PMU

Untersuchung des Vasa vasorums der großen humanen Beinvene für Herz by-pass Operationen - Vascular Corrosion casting
2014-2016
Bernd Minnich, Markus Herbst
Koop.Partner 1: Prof. Hölzenbein, Klinik f. Vaskuläre und Endovaskuläre Chirurgie, SALK - PMU
Koop.Partner 2: Prof. Stingl, 3. Anatomie, Karls-Universität Prag, CZ

Gefäßatlas des südafrikanischen Krallenfrosches Xenopus lavis - Vascular Corrosion casting
2011-2017
Alois Lametschwandtner, Bernd Minnich
FWF

Gefäßausgusspräparation BioTree Systems - Vascular Corrosion casting
2015-2016
Alois Lametschwandtner
Max Plank Institut für Biogeochemie Jena, Bundesforstamt Thüringer Wald, Thüringer Forst - Forschungsprojekt: BT_0610

Microvaskularization of murine organs - Vascular Corrosion casting
2015
Alois Lametschwandtner
Max Plank Institut für Biogeochemie Jena, Bundesforstamt Thüringer Wald, Thüringer Forst - Forschungsprojekt: BT_0610

Tumorvaskularisation II - Vascular Corrosion casting
2012-2014
Alois Lametschwandtner
Max Plank Institut für Biogeochemie Jena, Bundesforstamt Thüringer Wald, Thüringer Forst - Forschungsprojekt: BT_0610

NETs bei COPD
2011-2017
Stoiber, Krautgartner
Koop.Partner: Prof. Michael Studnicka, Pulmologie, SALK - PMU

Sacral versus pudendal neuromodulation
2014-2016
Krautgartner, Stoiber
Koop.Partner: Prof. Janetschek, Urologie, SALK - PMU

New phylogenetic features in Oligotrichea
2016-2017
Agatha
FWF - Forschungsprojekt: P28790
Maturation of the gastric microvasculature in Xenopus laevis (Lissamphibia, Anura) occurs at the transition from the herbivorous to the carnivorous lifestyle, predominantly by intussuceptive microvascular growth (IMG): a scanning electron microscope study of microvascular corrosion casts and correlative light microscopy.
2012
Lametschwandtner, A., Höll, M., Bartel, H., Anupunpisit, V. and B. Minnich
Anatomical Sciences International -Vol. 87: 88-100.
DOI 10.1007/s12565-011-0124-8

The microvascular anatomy of the trachea in adult Xenopus laevis Daudin (Lissamphibia; Anura): scanning electron microscopy of vascular corrosion casts and correlative light microscopy.
2012
Tangphokhanon W and Lametschwandtner A.
Anat Rec (Hoboken) 295: 1045-1052.

Morphometric SEM 3D analysis of microvascular networks and automated calculation of vessel angioarchitecture optimalities.
2012
Minnich, B., Margiol, S., Frysak, J. and E.W.N. Bernroider
In: FORMATEX, Current microscopy contributions to advances in science and technology, A. Méndes-Vilas and J. Diaz Alvarez (Eds). Vol. I, 191-199.
ISBN (13): 978-84-939843-5-9.

Ultrastructural characterization of cystic fibrosis sputum using atomic force and scanning electron microscopy.
2012
Manzenreiter R, Kienberger F, Marcos V, Schilcher K, Krautgartner WD, Obermayer A, Humi M, Stoiber W, Hector A, Griese M, Hannig M, Studnicka M, Vitkov L, Hartl D
J Cyst Fibros 11:84–92.

Microvascular anatomy of the esophagus in larval and adult Xenopus laevis Daudin: a scanning electron microscope study of microvascular corrosion casts and correlative light microscopy.
2013
Lametschwandtner, A., Bartel, H. and B. Minnich
Acta Zoologica, 7p. Vol.94: 447-453.
DOI: 10.1111/j.1463-6395.2012.00573.x.

Microvascular anatomy and histomorphology of extrapulmonary bronchi in adult Xenopus laevis Daudin (Lissamphibia; Anura) point to a role in aerial gas exchange – histomorphology of tissue sections and scanning electron microscopy of vascular corrosion casts.
Article first published online: 15 FEB 2013
Tangphokhanon W., Lametschwandtner, A.
Acta Zool (Stockh.).
DOI: 10.1111/azo.12025

The microvascular anatomy of the lung in adult Xenopus laevis Daudin (Lissamphibia; Anura): scanning electron microcopy of vascular corrosion casts and correlative light microscopy.
2013
Tangphokhanon W., Lametschwandtner A.
Transactions of the Royal Society of South Africa, 68:1, 11-23
DOI: 10.1080/0035919X.2012.747188

Microvascular architecture of mouse urinary bladder described with vascular corrosion casting, light microscopy, SEM, and TEM.
2013
Hossler FE, Lametschwandtner A, Kao R, Finsterbusch F.
Microsc Microanal. 1428-35.
DOI: 10.1017/S143192761301341X.

Microvascularization and histomorphology of lateral line organs in adult Xenopus laevis.
2014
Gerlach N, Minnich B, Lametschwandtner A.
J Morphol. 275:497-503

Characterization of the Vasa vasorum in the human saphenous vein: A scanning electron microscopy and 3D-morphometry study of vascular corrosion casts.
2014
Herbst, M., Hölzenbein, Th. and B. Minnich
Microscopy & Microanalysis, Vol.20, Issue 04, 1120-1133;
DOI 10.1017/S1431927614001287.

Histomorphology and microvasculature of extrahepatic bile ducts, extrapancreatic ducts and choledocho-pancreatic ducts in the adult African Clawed Toad, Xenopus laevis: Histomporphology and scanning electron microscopy of microvascular corrosion casts.
2015
Lametschwandtner, A., Bartel, H., Radner, C. and B. Minnich
Transactions of the Royal Society of South Africa
DOI 10.1080/0035919X.2015.1078263.

The effects of ▲9-Tetrahydrocannabinole treatment on gonadal micro-vascularization and affected fertility examined by SEM and 3D-morphometry
2015
Erlbacher, KMT. and B. Minnich
Journal of Physics: Conference Series 664: 012041
DOI 10.1088/1742-6596/644/1/012041.

Porcine liver vascular bed in Biodur E20 corrosion casts.
2016
Eberlova L, Liska V, Mirka H, Gregor T, Tonar Z, Palek R, Skala M, Bruha J, Vycital O, Kalusova K, Haviar S, Kralickova M, Lametschwandtner A.
Folia Morphol (Warsz) 75:154-161

Microvascularization of the pancreas in larval and adult Xenopus laevis – Histomorphology and scanning electron microscopy of vascular corrosion casts.
2016
Höpner J.P., Lametschwandtner A.
Arch Histol. Cytol 76: 35-52

Vascular architecture of the carotid labyrinth in larval and adult Xenopus laevis – Histomorphology and scanning electron microscopy of vascular corrosion casts.
2016
Deutschmann, C., Minnich, B. and A. Lametschwandtner
Arch.Histol.Cytol., 77: 1-11.

Microvascularization of the Spleen in Larval and Adut Xenopus laevis: Histomorphology and Scanning Electron Microscopy of Corrosion Casts.
2016
Lametschwandtner, A., Radner, Ch., and B. Minnich
J. Morphology,
DOI 10.1002/jmor.20595.

Transcutaneous vaccination via laser microporation.
2010
Weiss R, Hessenberger M, Kitzmüller S, Bach D, Weinberger EE, Krautgartner WD, Hauser-Kronberger C, Malissen B,Boehler C, Kalia YN, Thalhammer J, Scheiblhofer S.
Journal of Controlled Release, 162(2): Elsevier Verlag, 391-399

Transcutaneous immunotherapy via laser-generated micropores efficiently alleviates allergic asthma in Phl p 5-sesitized mice.
2012
Bach D, Weiss R, Hessenberger M, Kitzmüller S, Weinberger EE, Krautgartner WD, Hauser-Kronberger C, Boehler C, Thalhammer J, Scheiblhofer S.
Allergy.
DOI: 10.1111/all.12005.

Neutrophils express distinct RNA receptors ina Non-canonical way.
2012
Berger M, Hsien C-Y, Bakele M, Marcos V, Rieber N, Kormann M, Mays L, Hofer L, Neth O, Vitkov L, Krautgartner WD, Schweinitz D, Kappler R, Hector A, Weber A and Hartl D.
J Biol Chem. 287(23):19409-17.
DOI 10.1074/jbc.M112.353557.

Neutrophil extracellular traps are a main constituent of sputum from patients with chronic obstructive pulmonary disease.
2014
Obermayer A, Stoiber W, Krautgartner WD, Klappacher M, Kofler B, Steinbacher P, Vitkov L, Grabcanovic-Musija F, Studnicka M PLoS One 9: e97784.

Neutrophil extracellular trap (NET) formation characterises stable an exacerbated COPD and correlates with airflow limitation.
2015
Grabcanovic-Musija F, Obermayer A, Stoiber W, Krautgartner WD, Steinbacher P, Winterberg N, Bathke AC, Klappacher M, Studnica M
Resp Res 16:59

The initial inflammatory response to bioactive implants is characterized by NETosis.
2015
Vitkov L, Krautgartner W-D; Obermayer A; Stoiber W; Hannig M; Klappacher M; Hartl D
PloS ONE 10(3): e0121359
DOI:10.1371/journal.pone.0121359

Intraclass evolution and classification of the Colpodea (Ciliophora).
2011
Foissner W., Stoeck T., Agatha S. & Dunthorn M.
J. Eukaryot. Microbiol. 58: 397 -415

On the nature of tintinnid loricae (Ciliophora: Spirotricha, Tintinnina): a histochemical, enzymatic, EDX, and high-resolution TEM study.
2012
Agatha S. & Simon P.
Acta Protozool. 51: 1 -19 + Supplement 1 -4

Reconciling cladistic and genetic analyses in choreotrichid ciliates (Ciliophora, Spirotricha, Oligotrichea).
2012
Agatha S. & Strüder-Kypke M.C.
J. Eukaryot. Microbiol. 59: 325 -350

Systematics and evolution of tintinnid ciliates.
2013
Agatha S. & Strüder-Kypke M.C.
In: Dolan J.R., Montagnes D.J.S., Agatha S., Coats W.D. & Stoecker D.K. (eds.): The Biology and Ecology of Tintinnid Ciliates. Wiley-Blackwell: 42 -84.

The tintinnid lorica
2013
Agatha S., Laval-Peuto M. & Simon P.
In: Dolan J.R., Montagnes D.J.S., Agatha S., Coats W.D. & Stoecker D.K. (eds.): The Biology and Ecology of Tintinnid Ciliates. Wiley-Blackwell: 17 -41.

The Biology and Ecology of Tintinnid Ciliates
2013
Dolan J.R., Montagnes D.J.S., Agatha S., Coats W.D. & Stoecker D.K.
i -viii + 1 -296

Redescription of Strombidium coronatum (Leegaard, 1915) Kahl, 1932 (Ciliophora, Spirotricha) based on live observation, protargol impregnation, and scanning electron microscopy.
2014
Agatha S.
Acta Protozool. 53: 287 -294.

What morphology and molecules tell us about the evolution of Oligotrichea (Protista, Ciliophora).
2014
Agatha S. & Strüder-Kypke M.C.
Acta Protozool. 53: 77 -90 + Supplement 1 -19.

Updating biodiversity studies in loricate protists: the case of the tintinnids (Alveolata, Ciliophora, Spirotrichea).
2016
Santoferrara L.F., Bachy C., Alder V.A., Gong J., Kim Y.-O., Saccà A., Silva Neto I. D. da, Strüder-Kypke M. C., Warren A., Xu D., Yi Z., & Agatha S.
J. Eukaryot. Microbiol. 63: 651 -656 + Supplement 1 -8.